Coding

Part:BBa_K4131004:Design

Designed by: Chun Ying Yu   Group: iGEM22_CCU_Taiwan   (2022-10-09)


pfa B gene (Moritella marina), codon optimized for E. Coli expression.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1991
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1267
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is codon optimized for E.Coli expression, and the restriction enzyme sites of XbaI, SpeI, EcoRI, PstI, and NotI are eliminated.


Source

The sequence is extracted from the region 1629940-1632591 in Moritella marina genome (WP_019440098.1)

References